Estimation of serum creatinine by jaffe's method pdf

The two manual methods were examined with respect to a number. Abstract estimation of serum creatinine by routine jaffes method and by dry chemistry in icteric and hemolytic serum samples authors. Methods for quantitative creatinine determination current protocols. Serum creatinine determined by jaffe, enzymatic method. Serum creatinine varies with the subjects age, body weight, and sex.

Although the manuscript describes the nature of a precipitate and does not deal with an analytical assay, jaffes landmark paper elucidated the basic principles of the creatinine determination method originally developed by otto folin, which became immensely popular and has. Considering the length of time that has elapsed since jaff6 1. An experiment performed by toora and rajagopal 2002 indicated that after an incubation duration of approximately 15 minutes, at a normal temperature the orange color developed. From the harriman research laboratory, the roosevelt hospital, new york. Several approaches have been suggested to overcome this interference. Experiments weredesigned tohelpelucidate theeffectofketone interference inserum andurine creatinine estimations. Quantitative turbidimetric immunoassay for estimation of cystatin c in human serumplasma. The ratio of urine albumin to urine creatinine is used to predict. Method comparison14 serum patient samples were used. Routine clinical biochemistry laboratories use several methods for the estimation of serum and urinary concentrations of creatinine, most of which are based on the jaffes reaction described first by jaffe in 1886. Full text full text is available as a scanned copy of the original print version.

The calibration of jaffe method for serum creatinine using one serum based standard complemented with artificial matrix compensating standards jaffeinterfering substances allows twopoint. Determine the creatinine value of 2 unknown samples with 90% accuracy. Serum samples previously analyzed by modified jaffes kit method in the range of 54. Newer analytical methods are introduced in clinical biochemistry laboratory for the purpose of improvement of quality, automation, to reduce the cost or to simply measure a new analyte. For normal individual the reference interval for the ratio is between 12 and 20 mg ureamg creatinine. The jaffe and enzymatic methods are the two most common methods for measuring serum creatinine. Using jaffes reaction method, when creatinine is exposed to the picric acid which is in an alkaline substance, it quantitatively produces an orange color. Pdf determination of serum creatinine by jaffe method and how to. Creatinine measurement is useful in the diagnosis and treatment of renal diseases, in monitoring renal dialysis, and as a calculation basis for other urinary analytes e. Acetohexamide also gives falsely elevated serum creatinine in jaffes method 14. Method comparison between the enzymatic creatinine method y and jaffes kinetic method x by linear regression analysisgave the followingequation for all groups together n318gave the following equation. The interference of bilirubin in kinetic alkaline picrate creatinine assays jaffe has been widely known and described in the literature.

Original article effects of glucose and bilirubin on. The reaction has been brought to use for the measurement of creatinine by folin and wu in 1919. Creatinine was analyzed by both jaffes kinetic and the enzymatic method. The determination of creatinine in serum or urine is car ried out by jaffes reaction where creatinine reacts. It is sometimes low in subjects with relatively small muscle mass, cachetic patients, amputees, and in older persons. However, these normal ranges may be different in different labs since all of them take different normal ranges. Urine creatinine is then estimated by using jaffes method. Many publications describe a negative interference of bilirubin in jaffe creatinine assays 2, 3. Falsely decreased creatinine results can have farreaching implications on the treatment of patients. The jaffe reaction is a colorimetric method used in clinical chemistry to determine creatinine levels in blood and urine. Positive and negative false estimates of serum creatinine letter to the editor dopamine in both the methods but dobutamine caused positive interference with jaffes method but negative interference with enzymatic methods. A serum creatinine level that would usually be considered normal does not rule out the presence of.

Due to the low value, an accurate measurement is warranted in the assessment of renal dysfunction. The enzymatic method is based on the established determination of. The unit in which creatinine is measured is millimeter per second. Serum creatinine jaffe method chemistry libretexts. Spectrophotometric assay of creatinine in human serum. In 1886, max jaffe discovered a reaction of creatinine with picric acid in an. Without taking into consideration the acidic nature of standard, protein free filtrate pff of serum and urine, i % picric acid and 0.

Measurement of creatinine in serum is a renal func tion tese. Serum creatinine was determined according to the kinetic jaffes method, using an automated biochemical analyzer olympus au400 and commercially available assay kits by the same manufacturer. Creatinine reacts with picrate ion formed in alkaline medium to develop a redorange colour. In this article three different creatinine kits jaffes kinetic method have been tested for bilirubin interference and its rectification using two simple approaches.

Effects of bilirubin interference in the roche kinetic. Let us now go through the step by step procedure of performing this test and find the presence of serum creatinine by jaffes method. Serum creatinine determination is mainly used for the diagnosis of renal disease. Estimation of serum creatinine by routine jaffes method and by. Explain why a filtrate preparation is required in procedures of this type.

The aim of this paper was to compare precision of serum creatinine estimation by modified jaffes kinetic method and enzymatic method. Increase plasma urea with normal creatinine gives rise to high ratio and seen in prerenal states. In the jaffe method, creatinine forms a yelloworange complex with picric acid in alkaline solution. So, in higher dose exposure of streptomycin to human subjects serum urine creatinine estimation by jaffe method has to be interpreted in terms of available streptomycin concentration.

The obtained results indicated a linear correlation with the diagnostic kit method. Estimation creatinine by folins method jaffes reaction. Estimation of serum creatinine by routine jaffes method and by dry. Creatinine may be measured in both serum and urine. Pdf the calibration of jaffe method for serum creatinine using one serumbased standard complemented with artificial matrix compensating standards. The jaffes method of serum creatinine deter mination in based on the principle that picric acid in an alka line reacts with creatinine to form a yellowred complex with the alkaline picrate 4. Make sure that you follow each and every step very carefully in order to have a successful test.

However, his serum urea was within normal limits but serum sodium was too high. Positive and negative false estimates of serum creatinine. The jaffes method for serum creatinine determination is based on the principle that picric acid in an alkaline medium reacts with creatinine to form a yellowred complex with the alkaline picrate 4. Estimation serum creatinine by folins method jaffes. Serum creatinine levels were measured by means of an isotope dilution mass spectrometry idms traceable assays both at pgi modified jaffes method using cobas c111 autoanalyzer, roche diagnostics limited and in a central lab in japan enzymatic creatinine assay using hitachi creatinine autoanalyzer, model 7170, hitachi. The measurement of urea nitrogencreatinine ratio can be used as crude discrimination between prerenal and postrenal azotemia. Estimation of serum creatinine by routine jaffes method. The basis of nearly all methods of estimation is the colour of the alkaline creatinine picrate.

Serum glucose and protein had a significant effect on jaffe method, but to a less significant extend on enzymatic method. Pdf the calibration of jaffe method for serum creatinine using one serum based standard complemented with artificial matrix compensating standards. Colorimetric estimation of creatinine using the alkaline picrate method. The colour produced from the sample is then compared in a colorimeter at wavelength of 505 nm with that produced by a known amount of creatinine under the same condition. Taussky from the department of obstetrics and gynecology, cornell university medicat college and the new york hospital, new york received for. As for uremic patients, in whom serum creatinine level is high and many of them suffered from dm as their cause of renal failure, serum creatinine can be either under. Roscoe from the department of medicine, manchester university received for publication february 17, 1953 although the clearance of endogenous creatinine as a measure of the glomerular filtration rate has been muchcriticized, it remains the only possible measure where repeated or continuous investiga. Over the years, the jaffes assay has progressed through many phases. Serum creatinine a blood measurement is an important indicator of renal health because it is an easily measured byproduct of muscle metabolism that is excreted unchanged by the kidneys method. Interference with creatinine estimation by impenem. List substances that cause interferance with the alkaline picrate method of creatinine analysis. Four serum specimens wereanalyzed bythethreecreatinine methodsbefore and.

Since then, jaffes reaction has been used for estimation of creatinine in serum and urine sample. Theconcomitant urinevalues tended toshowaslightdecrease. So if, actual concentration of creatinine is 53 micromoleslitre, then in presence of 10 mgml streptomycin in serum, the apparent concentration would be. Creatinine determination according to jaffewhat does it stand for. Diagnosis and management pdf 8th edition direct link medical students corner on daviss drug guide for nurses 16th edition pdf free download. Bilirubin interference in serum creatinine estimation by. Analytical recovery was evaluated by adding stock creatinine solution to serum samples and analyzing the concentration.

The determination of serum creatinine concentrations by enzymatic method on backman coulter au 480 and modified jaffes kinetic method was performed on siemens dimension clinical chemistry. Serum creatinine determination is mainly used for the diagnosis of renal diseases. For more than 90 years, this method is in use for the measurement of creatinine concentration due to its simplicity and low cost. Measurement of creatinine by jaffes reaction determination of. Creatinine determination according to jaffewhat does it. Estimation of renal function in patients with chronic. Estimation serum creatinine by folins method jaffes reaction may 29. Creatinine in serum or urine is determined by jaffes reaction where creatinine produces quantitatively an orange color with picric acid in alkaline medium.

Modified jaffes kinetic method for the determination of creatinine in serum and urine pdf. The negative interference by bilirubin in serum creatinine estimation by jaffes kinetic method is well known. Get a printable copy pdf file of the complete article. Although these tests are not expensive, they are highvolume tests and the savings could. So, this was all from the creatinine clearance test. Pdf determination of serum creatinine by jaffe method. Also described is a high performance liquid chromatography hplc method that separates.

Padmanabhan preeti, and jangle n suresh background. Although the manuscript describes the nature of a precipitate and does not deal with an analytical assay, jaffes landmark paper elucidated the basic principles of the creatinine determination method originally developed by otto folin, which became immensely popular and has easily. Estimation of serum creatinine,urine creatinine, and. Recently, the serum creatinine of a patient admitted in our intensive coronary care unit was found to be 7. There are major analytical problems associated with the use of the jaffes reaction, in. The concentration of the dye formed is proportional to creatinine concentration. Estimation of creatinine by the jaffe reaction clinical chemistry. In 1886, max jaffe discovered a reaction of creatinine with picric acid in an alkaline environment.